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Failure to detect Chlamydia pneumoniae DNA in cerebral aneurysmal sac tissue with two different polymerase chain
1Department of Neurosurgery, Ege University School of Medicine, Izmir, Turkey.
Objective:
Chlamydia pneumoniae (C pneumoniae) is a common cause of a usually mild, community acquired pneumonia. This organism, however, can spread from the respiratory tract into other parts of the body and has been detected in up to 70% of atheromatous lesions in blood vessels. Although the exact mechanism of the C Pneumoniae contribution to the pathogenesis of atherosclerosis remains unknown, prophylactic antibiotic trials are planned for people at high risk for coronary disease.
Method:
In this study the authors aimed to investigate C pneumoniae DNA content in the cerebral aneurysmal sac tissue with the aid of polymerase chain reaction (PCR) method. C pneumoniae DNA was searched in 15 surgically clipped and removed aneurysmal sac tissue and in two tumour (an ependymoma of the fourth ventricle and a craniofaringoma) samples by touchdown enzyme time release PCR (TETR PCR) targeting 16S rRNA gene and by nested PCR targeting ompA gene.
Results:
Both PCR methods were sensitive to detect in C pneumoniae 4x10(-2) genomes. C pneumoniae DNA was not detected in any of the 17 sample tissues of these patients.
Conclusion:
The contribution of C pneumoniae in the development of intracranial aneurysms cannot be excluded despite the results of this study. Further studies on the possible role of C pneumoniae or any other micro-organisms in the pathogenesis of aneurysms should be performed.
Insights
This study investigated Chlamydia pneumoniae DNA in cerebral aneurysm tissues using PCR, finding no evidence of the bacteria. Further research is needed to explore the role of C. pneumoniae in aneurysm development.
Area of Science:
- Infectious Disease
- Neurology
- Cardiovascular Research
Background:
- Chlamydia pneumoniae is a common respiratory pathogen.
- C. pneumoniae DNA has been found in atherosclerotic lesions, suggesting a role in cardiovascular disease.
- Its role in intracranial aneurysms is currently unknown.
Purpose of the Study:
- To investigate the presence of Chlamydia pneumoniae DNA in cerebral aneurysmal sac tissue.
- To determine if C. pneumoniae contributes to the pathogenesis of intracranial aneurysms.
Main Methods:
- Utilized polymerase chain reaction (PCR) methods, including touchdown enzyme time release PCR (TETR PCR) and nested PCR.
- Analyzed 15 surgically removed aneurysmal sac tissues and 2 tumor samples.
- Targeted the 16S rRNA gene and ompA gene for C. pneumoniae detection.
Main Results:
- Both PCR methods demonstrated high sensitivity, capable of detecting C. pneumoniae at 4x10(-2) genomes.
- Chlamydia pneumoniae DNA was not detected in any of the 17 analyzed human tissue samples.
Conclusions:
- This study did not find evidence of C. pneumoniae in cerebral aneurysms.
- The potential contribution of C. pneumoniae to intracranial aneurysm development cannot be definitively ruled out.
- Further investigation into the role of C. pneumoniae and other microorganisms in aneurysm pathogenesis is warranted.