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[Antibiotic interaction with proteolytic enzymes]
Abstract:
The effect of penicillin, tetracycline, aminoglucozide antibiotics and streptomycin on BAEE-esterase activity of trypsin was studied. It was found that benzylpenicillin in amounts of 50, 100 and 300 mg, ampicillin in an amount of 25 mg, methicillin in an amount of 12 mg and tetracycline in an amount of 2.5 mg as calculated per 1 mg of trypsin had no effect in vitro on the esterase activity of the enzyme. Neomycin, kanamycin and streptomycin in amounts of 5, 10, 100 or 300 mg per 1 mg of trypsin catalyzed splitting of BAEE by trypsin. When the antibiotics were added to the bile, its esterase activity increased. Preliminary intramuscular administration of trypsin and kanamycin to the rats had no effect on the ampicillin levels in the blood serum and brain and did not affect the permeability of the hemato-encephalic barrier as compared to the use of trypsin alone.
Insights
Certain antibiotics like neomycin, kanamycin, and streptomycin enhance trypsin
Area of Science:
- Biochemistry
- Pharmacology
Context:
- Investigating the enzymatic activity of trypsin.
- Assessing the impact of various antibiotics on biological systems.
Purpose:
- To determine the in vitro effect of penicillin, tetracycline, aminoglycoside antibiotics, and streptomycin on the BAEE-esterase activity of trypsin.
- To evaluate the influence of these antibiotics on bile's esterase activity.
- To examine the effect of combined trypsin and kanamycin administration on ampicillin levels and blood-brain barrier permeability in rats.
Summary:
- Benzylpenicillin, ampicillin, methicillin, and tetracycline did not inhibit trypsin's in vitro esterase activity.
- Neomycin, kanamycin, and streptomycin significantly catalyzed the BAEE-esterase activity of trypsin.
- Antibiotics increased bile's esterase activity, and combined trypsin-kanamycin treatment in rats did not alter ampicillin levels or blood-brain barrier permeability.
Impact:
- Highlights specific antibiotic classes that modulate trypsin activity, relevant for drug interactions and enzyme kinetics.
- Suggests potential applications or contraindications for using trypsin with certain antibiotics.
- Provides insights into the pharmacokinetic and pharmacodynamic interactions of antibiotics and enzymes in vivo.