[Antibiotic interaction with proteolytic enzymes].

Area of Science:

• Biochemistry
• Pharmacology

Context:

• Investigating the enzymatic activity of trypsin.
• Assessing the impact of various antibiotics on biological systems.

Purpose:

• To determine the in vitro effect of penicillin, tetracycline, aminoglycoside antibiotics, and streptomycin on the BAEE-esterase activity of trypsin.
• To evaluate the influence of these antibiotics on bile's esterase activity.
• To examine the effect of combined trypsin and kanamycin administration on ampicillin levels and blood-brain barrier permeability in rats.

Summary:

• Benzylpenicillin, ampicillin, methicillin, and tetracycline did not inhibit trypsin's in vitro esterase activity.
• Neomycin, kanamycin, and streptomycin significantly catalyzed the BAEE-esterase activity of trypsin.
• Antibiotics increased bile's esterase activity, and combined trypsin-kanamycin treatment in rats did not alter ampicillin levels or blood-brain barrier permeability.

Impact:

• Highlights specific antibiotic classes that modulate trypsin activity, relevant for drug interactions and enzyme kinetics.
• Suggests potential applications or contraindications for using trypsin with certain antibiotics.
• Provides insights into the pharmacokinetic and pharmacodynamic interactions of antibiotics and enzymes in vivo.