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Updated: Jul 20, 2026
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Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue
Published on: June 18, 2014
Cell analysis with the new Leipzig high-energy ion nanoprobe
J Osterreicher1, J Vogt, J M Tanner
1Universitat Leipzig, Fakultat fur Physik und Geowissenschaften, Linnestr, 5, 04103 Leipzig, Germany. osterreicher@pmfhk.cz
Radiatsionnaia Biologiia, Radioecologiia
|May 21, 2003
Summary
The Leipzig Ion Projection Spectrometry Of Nanoscale (LIPSION) facility uses ion beams for high-resolution microscopy and tomography. Current research focuses on chondrocytes and endothelial cells, with future work on living cells.
Area of Science:
- Physics
- Materials Science
- Biology
Background:
- The Leipzig Ion Projection Spectrometry Of Nanoscale (LIPSION) facility has been operational since 1998, utilizing a 3.5 MV SINLETRON accelerator for ion beam generation.
- The facility employs a magnetic scanning system for precise beam movement and achieves high resolution (41 ± 4 nm in low current mode).
Purpose of the Study:
- To detail the capabilities of the LIPSION ion nanoprobe facility.
- To highlight current biomedical research applications, including microscopy and tomography of chondrocytes and endothelial cells.
- To introduce advancements for future research involving single living cell irradiation.
Main Methods:
- Utilizes a 3.5 MV SINLETRON accelerator to supply H+ or He+ ion beams.
- Employs magnetic scanning for focused beam movement across samples.
- Integrates electron, energy dispersive X-ray, and particle detectors for simultaneous analysis via Particle-Induced X-ray Emission (PIXE), Rutherford Backscattering (RBS), and Scanning Transmission Ion Microscopy (STIM).
- Incorporates a goniometer for channeling measurements in single crystals.
Main Results:
- Achieves resolutions of 41 ± 4 nm (low current) and 300 nm (at 5 pA).
- Current biomedical research focuses on microscopy and tomography of pig chondrocytes and fixed human umbilical vein endothelial cells (HUVEC).
Conclusions:
- The LIPSION facility provides advanced capabilities for nanoscale analysis.
- Ongoing research demonstrates its utility in cell biology, with planned enhancements for live-cell studies.
