Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hardware (DNA) circuits.

Richard D'Ari1, René Thomas

  • 1Institut Jacques-Monod (CNRS, universités Paris-6 & 7), 2, place Jussieu, 75251 Paris, France. dari@ijm.jussieu.fr

Comptes Rendus Biologies
|May 21, 2003
PubMed
Summary

Scientists developed a novel genetic control circuit enabling precise on/off gene expression. This system utilizes a positive feedback loop, offering two stable states for gene regulation and control.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the impact of academic coaching interventions on student outcomes in graduate healthcare and medical education: a systematic scoping review.

Medical education online·2025
Same author

Comparison of face to face and online delivery of a dementia-specific experiential learning activity.

Gerontology & geriatrics education·2024
Same author

Facilitators and challenges experienced by nursing homes enrolling in the CDC national health care safety network.

American journal of infection control·2020
Same author

Opportunities to Improve Informed Consent with AHRQ Training Modules.

Joint Commission journal on quality and patient safety·2018
Same author

Pain Management in the Pediatric Palliative Care Population.

Journal of nursing scholarship : an official publication of Sigma Theta Tau International Honor Society of Nursing·2018
Same author

Merci François Jacob.

Research in microbiology·2014

Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Genetic Engineering

Background:

  • Gene expression control is fundamental in biological research and biotechnology.
  • Developing precise and switchable genetic circuits remains a key challenge.

Purpose of the Study:

  • To design and present a novel genetic control circuit for inducible gene expression.
  • To enable researchers to control gene expression levels at will within an organism.

Main Methods:

  • Engineered a positive feedback loop using the bacteriophage lambda CII protein.
  • Designed the structural gene for CII to be dependent on CII for its own expression.
  • Incorporated additional circuits for switching between expression states.

Main Results:

  • The positive feedback loop established two stable steady states: cII ON or cII OFF.
  • Downstream genes without promoters followed the expression state of cII.
  • Demonstrated the ability to switch between ON and OFF states using auxiliary circuits.

Conclusions:

  • The presented genetic circuit provides a robust mechanism for binary gene control.
  • This system offers a powerful tool for synthetic biology applications requiring precise gene regulation.
  • The switchable nature of the circuit allows for dynamic control of gene function.

Related Experiment Videos