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Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis
Joaquin J Jimenez1, Wenche Jy, Lucia M Mauro
1Wallace H Coulter Platelet Laboratory, Division of Hematology/Oncology, University of Miami School of Medicine, 1600 NW 10th Ave, Mail Code R36A, Miami, FL, USA. jjimenez@med.miami.edu
Thrombosis Research
|May 22, 2003
Summary
Endothelial cells (EC) release distinct endothelial microparticles (EMP) during activation versus apoptosis. Analyzing these EMP signatures offers insights into endothelial health.
Area of Science:
- Cardiovascular Biology
- Cellular Biology
- Biomarker Discovery
Background:
- Endothelial cells (EC) release endothelial microparticles (EMP) during cellular activation and apoptosis.
- EMP are cell-derived vesicles with potential roles as biomarkers.
Purpose of the Study:
- To compare the antigenic expression of EMP released from EC during activation versus apoptosis.
- To investigate differences in EMP profiles across various EC types.
Main Methods:
- Utilized three EC types: renal microvascular (MiVEC), brain MiVEC, and coronary macrovascular (MaVEC).
- Induced activation with TNF-alpha and apoptosis via growth factor deprivation.
- Assessed antigen expression on EMP and EC using flow cytometry, including constitutive markers, inducible markers, and annexin V binding.
Main Results:
- Apoptosis led to increased constitutive markers (e.g., CD31, CD105) on EMP and increased annexin V binding.
- Activation resulted in elevated inducible markers (e.g., CD62E, CD54, CD106) on both EMP and EC.
- Coronary MaVEC released fewer EMP compared to MiVEC.
Conclusions:
- EC release qualitatively and quantitatively distinct EMP during activation compared to apoptosis.
- EMP phenotypic signatures can provide clinically relevant information about endothelial status.