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Virus inactivation by high intensity broad spectrum pulsed light
1Bio Products Laboratory, Research and Development Department, Dagger Lane, Elstree, WD6 3BX, Herts, UK. peter.roberts@bpl.co.uk <peter.roberts@bpl.co.uk>
Journal of Virological Methods
|May 22, 2003
Summary
High-intensity pulsed light effectively inactivates most viruses. However, protein presence reduces efficacy, with some viruses showing resistance, impacting its use in biological products.
Area of Science:
- Microbiology
- Biotechnology
- Photochemistry
Background:
- Viruses pose a significant risk to the safety of therapeutic biological products.
- Effective methods for virus inactivation are crucial for ensuring product safety.
Purpose of the Study:
- To investigate the efficacy of high-intensity broad-spectrum pulsed light (PureLight) for inactivating various viruses.
- To assess the impact of protein presence on the virus inactivation process.
Main Methods:
- Exposure of enveloped and non-enveloped viruses to high-intensity pulsed light.
- Testing inactivation in phosphate-buffered saline and in the presence of fetal calf serum.
- Quantification of virus log reduction post-treatment.
Main Results:
- A dose of 1.0 J/cm² inactivated >4.8–7.2 log of all tested viruses in saline.
- In 5% fetal calf serum, higher doses were needed, and some viruses (HSV-1, BPV, SV40) showed relative resistance.
- Inactivation ranged from 5.0–6.4 log at 2.0 J/cm² in serum, but specific viruses had lower reductions.
Conclusions:
- High-intensity pulsed light is a potent virucidal agent.
- Protein content in biological samples can significantly reduce the effectiveness of this inactivation method.
- Further research is needed to optimize conditions for protein-rich biological products.