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Related Experiment Videos

Protease-activated receptor-2 signaling triggers dendritic cell development.

Ryan C Fields1, Jonathan G Schoenecker, Justin P Hart

  • 1Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.

The American Journal of Pathology
|May 22, 2003
PubMed
Summary

Serine proteases, acting through protease-activated receptor-2 (PAR-2), stimulate dendritic cell (DC) development from bone marrow progenitors. This discovery highlights a novel pathway for initiating adaptive immune responses.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Dendritic cells (DCs) are crucial antigen-presenting cells that regulate immune responses.
  • DC development from progenitor cells is accelerated by inflammation but the precise signals remain unclear.

Purpose of the Study:

  • To investigate the role of serine proteases and protease-activated receptor-2 (PAR-2) in dendritic cell development.

Main Methods:

  • Bone marrow progenitor cells were cultured with GM-CSF and IL-4.
  • The effects of serine proteases, a serine protease inhibitor (soybean trypsin inhibitor), and a PAR-2 agonist peptide were assessed.
  • Dendritic cell development was examined in bone marrow cultures from PAR-2-deficient mice.

Main Results:

  • A serine protease acting via PAR-2 was found to stimulate DC development from bone marrow progenitors.

Related Experiment Videos

  • Inhibition of serine proteases blocked DC development, an effect reversed by a PAR-2 agonist.
  • PAR-2-deficient mice showed impaired spontaneous DC development, but could be stimulated by inflammatory mediators.

    • Conclusions:

    • Endogenous serine proteases stimulate dendritic cell development in vitro.
    • Serine proteases acting through PAR-2 represent a potential mechanism for initiating adaptive immune responses in vivo.