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Published on: November 22, 2011
[The pathogenicity of HFRS patient's soft palate mucosa to suckling mice]
Objective:
To explore the pathogenic mechanism of hemorrhagic fever with renal syndrome(HFRS).
Methods:
2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft palate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.
Results:
Some of the mice developed acute disease and died. The autopsy analysis indicated that these mice showed pathological changes in microvascular system and parenchymatous tissues. HFRS virus RNA was detected in their brains, lungs and kidney tissues.
Conclusions:
These results indicate that HFRS virus exists in the tissue of petechial hemorrhagic spots on soft palate petechia of HFRS patients.
Insights
Hemorrhagic fever with renal syndrome (HFRS) virus was found in soft palate petechia of patients. This virus caused acute illness and pathological changes in mice, confirming its presence in these tissues.
Area of Science:
- Virology
- Pathology
- Infectious Diseases
Context:
- Hemorrhagic fever with renal syndrome (HFRS) is a serious zoonotic disease.
- Understanding the pathogenic mechanisms of HFRS is crucial for developing effective treatments.
- The precise origin and distribution of the HFRS virus within infected patients require further investigation.
Purpose:
- To investigate the pathogenic mechanism of HFRS.
- To determine the presence and distribution of HFRS virus in patient tissues.
- To establish an animal model for studying HFRS pathogenesis.
Summary:
- Inbred Balb/C mice were inoculated with tissue suspension from HFRS patient soft palate petechia.
- Mice developed acute disease, exhibiting pathological changes in microvasculature and parenchymal tissues.
- HFRS virus RNA was detected in the brains, lungs, and kidneys of affected mice using nested PCR.
Impact:
- This study confirms the presence of HFRS virus in the petechial hemorrhagic spots of the soft palate.
- The findings contribute to understanding HFRS virus dissemination and pathogenesis.
- The established mouse model can be utilized for further research into HFRS prevention and treatment strategies.
