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Related Experiment Videos

[Phenotypic variation in Actinobacillus actinomycetemcomitans].

Zhe-ling Wang1, Nobuko Maeda, Tomoko Ohshima

  • 1Beijing Institute of Dental Research, Beijing Hospital for Stomatology, Capital University of Medical Sciences, Beijing 100050, China.

Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology
|May 23, 2003
PubMed
Summary

Colony morphology variation in Actinobacillus actinomycetemcomitans (Aa) from rough to smooth was observed during laboratory subculturing. This study identified distinct intermediate and smooth colony phenotypes during this transition.

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Area of Science:

  • Microbiology
  • Bacteriology

Background:

  • Actinobacillus actinomycetemcomitans (Aa) is associated with juvenile periodontitis.
  • Understanding bacterial colony variation is crucial for laboratory diagnostics and strain characterization.

Purpose of the Study:

  • To investigate the morphological changes of Actinobacillus actinomycetemcomitans (Aa) from rough to smooth colonies.
  • To identify and characterize different colony phenotypes during laboratory cultivation.

Main Methods:

  • Bacterial strains were isolated from subgingival plaque of periodontitis patients.
  • Repeated subculturing on agar plates and in broth was performed over multiple generations.
  • Morphological changes of Aa colonies were observed on solid media and in liquid cultures.

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Main Results:

  • Smooth Aa colony variants were obtained through broth subculturing over 7-8 generations.
  • Colonies transitioned from small, adherent, rough phenotypes with star-like structures to larger, opaque, nonadherent smooth phenotypes.
  • Agar subculturing did not yield completely smooth variants, and distinct rough, opaque smooth, and translucent smooth morphologies were identified.

Conclusions:

  • The transition of Actinobacillus actinomycetemcomitans (Aa) from rough to smooth colony morphology is a gradual process.
  • Intermediate colony morphologies, including opaque and translucent smooth types, were observed during this variation.
  • Laboratory subculturing methods influence the observed colony morphology changes in Aa.