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[Fabrication of cartilage in predetermined shapes from human nasoseptal chondrocytes with tissue engineering method]
1Department of Otorhinolaryngology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. cuipc@fmmu.edu.cn
Objective:
To investigate the feasibility of fabricating a new cartilage in predetermined shapes with tissue engineering methods.
Methods:
Human nasoseptal chondrocytes were seeded onto a nonwoven mesh of polyglycolic acid(PGA) to form a cell-PGA construct. The construction was then configured in sheet and tube shapes, and implanted subcutaneously into the dorsa of 11 athymic mice. The specimens were harvested 4, 6, 8 weeks after implantation and subjected to gross morphologic and histologic analysis.
Results:
Gross observation showed that the predetermined sheet and tube shapes of new cartilage were formed. Histological observation demonstrated that new mature cartilages were formed in 6-week. A Masson's trichrome stain showed the interwining bands of collagen at the periphery of the cartilage. Staining of Safranin O evaluated that the new cartilage was bound of glycosaminoglycan. In the control group, the PGA of the specimens were completely absorbed at 6 weeks.
Conclusion:
Human nasoseptal chondrocytes-PGA construct could develop into a new cartilage in predetermined shapes in athymic mice.