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The divergence between human and baboon globin genes.

R Dalgleish, R Williamson, P Tolstoshev

    Biochimica Et Biophysica Acta
    |June 2, 1976
    PubMed
    Summary
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    Baboon globin complementary DNA (cDNA) was synthesized from messenger RNA (mRNA). Hybridization studies revealed minor globin gene divergence between humans and baboons, limiting baboon cDNA as a probe for human sequences.

    Area of Science:

    • Molecular Biology
    • Primate Genetics
    • Biochemistry

    Background:

    • Messenger RNA (mRNA) and complementary DNA (cDNA) are crucial for gene expression studies.
    • Understanding gene divergence between species aids in evolutionary and genetic research.

    Purpose of the Study:

    • To synthesize baboon globin cDNA.
    • To compare human and baboon globin mRNA and cDNA hybridization.
    • To assess the evolutionary divergence of globin genes between humans and baboons.

    Main Methods:

    • Complementary DNA (cDNA) synthesis using viral RNA-directed DNA polymerase from baboon globin mRNA.
    • Formation and analysis of homologous and heterologous hybrids between human and baboon mRNA and cDNA.
    • Comparison of hybridization extent and thermal stability (melting temperature).

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    Main Results:

    • Higher mRNA inputs were needed for heterologous hybridization saturation.
    • Heterologous hybrids exhibited a 5°C lower melting temperature than homologous hybrids.
    • Globin gene divergence between humans and baboons is less than predicted by third-position coding sequence changes.

    Conclusions:

    • Globin gene sequences show limited divergence between humans and baboons.
    • Baboon globin cDNA is generally not suitable as a probe for human globin mRNA or gene sequences.
    • This study provides insights into primate globin gene evolution.