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Development of murine embryos following electroporation
C A Schmotzer1, M E Dunlap-Brown, S P Butler
1Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0315, USA.
Purpose:
The objective was to establish the parameters for reversible electroporation of murine embryos.
Methods:
In Trial 1, murine presumptive zygotes received an electrical pulse of 5, 10, or 20-micros duration, and one of five voltages (100, 200, 250, 300, or 400 V). In Trial 2, embryo orientation within the electroporation chamber was evaluated with 250 or 400 V at a pulse period of 10 micros.
Results:
Presumptive zygotes that received 400 V at each pulse length and zygotes exposed to 20 micros at each voltage had the lowest embryonic development (P < 0.05). Presumptive zygotes that received 250 V had higher development compared to 400 V, irrespective of orientation (P < 0.01), but development was lower than the controls (P < 0.01).
Conclusions:
Electrical stimulation of presumptive zygotes can have a detrimental impact on early embryo development, but low amounts of stimulation may allow for potential gene transfer in transgenic experimentation.