Calculation of electrophoretic mobility in ternary solvent electrolyte systems.

Area of Science:

• Analytical Chemistry
• Separation Science
• Physical Chemistry

Background:

• Capillary electrophoresis (CE) is a powerful separation technique.
• Understanding electrophoretic mobility is crucial for optimizing CE methods.
• Salmeterol and phenylpropanolamine are compounds with potential pharmaceutical applications.

Purpose of the Study:

• To determine the electrophoretic mobility of salmeterol and phenylpropanolamine in capillary electrophoresis.
• To investigate the effect of varying buffer compositions (water, methanol, acetonitrile) on mobility.
• To evaluate a mathematical model for predicting electrophoretic mobility.

Main Methods:

• Capillary electrophoresis was employed to measure electrophoretic mobility.
• Acetate buffer systems with varying concentrations of water, methanol, and acetonitrile were utilized.
• Experimental data were used to validate a predictive mathematical model.

Main Results:

• Maximum electrophoretic mobilities for salmeterol and phenylpropanolamine were observed at specific water-methanol-acetonitrile ratios (5:50:45, v/v and 3:60:37, v/v, respectively).
• Minimum mobilities for both compounds occurred at a methanol-acetonitrile ratio of 30:70 (v/v).
• The developed mathematical model accurately reproduced the experimental mobility data with mean percentage deviations of 1-4%.

Conclusions:

• The composition of the capillary electrophoresis buffer significantly influences the electrophoretic mobility of salmeterol and phenylpropanolamine.
• A validated mathematical model can reliably predict electrophoretic mobility, facilitating method optimization.
• This research contributes to the advancement of analytical techniques for pharmaceutical compound analysis.