High-performance liquid chromatographic analysis of nitrendipine in human plasma using ultraviolet detection and...

Area of Science:

• Analytical Chemistry
• Pharmacokinetics
• Biomedical Analysis

Background:

• Accurate quantification of drugs in biological matrices is essential for pharmacokinetic studies.
• Existing methods may lack the specificity or sensitivity required for nitrendipine analysis.
• Nitrendipine's pharmacokinetic profile requires a reliable analytical method for therapeutic drug monitoring.

Purpose of the Study:

• To develop and validate a high-performance liquid chromatographic (HPLC) method for determining nitrendipine concentrations in human plasma.
• To establish an assay suitable for pharmacokinetic investigations of nitrendipine.
• To ensure the method's specificity, accuracy, and precision for biological sample analysis.

Main Methods:

• High-performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection at 238 nm.
• Sample preparation involved solid-phase extraction (SPE) using a C18 cartridge.
• Nimodipine served as the internal standard; the mobile phase consisted of a phosphate buffer and acetonitrile mixture.

Main Results:

• The developed HPLC-UV method demonstrated high specificity, accuracy, and precision for nitrendipine in human plasma.
• The assay achieved a detection limit of 2.0 ng/ml for nitrendipine.
• Coefficients of variation (CV) were below 16.5%, with no significant interference from endogenous plasma components.

Conclusions:

• The validated HPLC-SPE-UV method provides a robust and reliable means for quantifying nitrendipine in human plasma.
• This assay is suitable for use in pharmacokinetic studies to evaluate drug absorption, distribution, metabolism, and excretion.
• The method's performance characteristics support its application in clinical and research settings for nitrendipine analysis.