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Glutamate induced currents in isolated inner hair cells from guinea-pig cochlea
Takashi Kimitsuki1, Mitsuru Ohashi, Yuki Wada
1Department of Otorhinolaryngology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. kimituki@fc.miyazaki-med.ac.jp
Brain Research
|May 24, 2003
Abstract:
We investigated the direct action of glutamate (Glu) on the membrane current of isolated inner hair cells of guinea-pig cochlea. Glu elicited inward currents at a holding potential of -70 mV. Eight of 13 cells showed a steady inward current, while five of 13 cells showed a fast and rapidly desensitized current. I-V relationships demonstrated that the reversal potential of Glu-induced current was near 0 mV. Glu-induced currents were dose-dependent, where the half maximum concentration (K(d)) was 41 microM and Hill coefficient (n) was 1.75.