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Updated: Aug 15, 2026

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
Published on: October 8, 2013
[Floating culture of human nasal glandular cells]
1Department of Otorhinolaryngology Head and Neck Surgery, Third Clinical College of Bethune University of Medical Sciences, Changchun 130031.
Objective:
To establish a floating cultural model of human nasal glandular (HNG) cells.
Methods:
HNG cells were cultured on floating collagen gels.
Results:
Cultured HNG cells incubated with monoclonal antibody for cytokeratin stained positively. After two-week culture, the cells stained blue by AB-PAS staining. Transmission electron microscopy showed that the cells exhibited numerous microvilli on their surfaces and had numerous secretary granules. They were well polarized and interconnected by junctional complexes. Cultured HNG cells possessed typical characteristics of normal epithelium.
Conclusion:
The floating cultural model of HNG was successfully established.
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