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[The expression of Ras protein in human middle ear cholesteatoma]
1Department of Otorhinolaryngology, Second Affiliated Hospital of Hubei Medical University, Wuhan 430071.
Objective:
To evaluate the possible role of Ras protein in acquired middle ear cholesteatoma.
Methods:
The specimens from the acquired middle ear cholesteatoma tissue of 22 cases and the normal external ear skin of 10 cases were examined by immunohistochemical S-P method and computer image analysis.
Results:
All epithelial layers of cholesteatoma revealed a relatively abundant expression of Ras protein, among which 15 cases showed an inner plasma membrane expression, 5 cases a cytoplasm and membrane expression, and 2 cases cytoplasm expression only. In contrast, the normal external ear skin revealed a sparse distribution of Ras protein in the inner plasma membrane of epithelial cells with a more intensely staining in the basal layer. The integral absorbency of Ras protein in the two types of tissues were 0.870 and 0.463 respectively with a significant difference between them.
Conclusion:
There is an aberrant regulation of Ras protein in cholesteatoma. Ras protein plays a pivotal role during the aberrant growth and the differentiation of cholesteatoma.