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Updated: Aug 11, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Published on: November 22, 2014
Chemical RNA labeling without 3' end bias using fluorescent cis-platin compounds
R Hagedoorn1, R Joseph, S Kasanmoentalib
1Leiden University Medical Center, Leiden, The Netherlands.
Abstract:
Recently, fluorescent, monofunctional cis-platin derivatives have been developed to chemically label nucleic acids for use in fluorescent hybridization assays. Here we show by hybridizations to microarrays containing oligonucleotide probes for the 3' ends, middle parts, and 5' ends of mRNAs, that this labeling methodology bypasses the problem of the 3' end bias that is characteristic of the conventional enzymatic oligo(dT)-primed, reverse transcription labeling of mRNAs.
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