Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proteins modulating TRP channel function.

Christian Harteneck1

  • 1Institut für Pharmakologie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Thielallee 69-73, 14195 Berlin, Germany. hartenek@zedat.fu-berlin.de

Cell Calcium
|May 27, 2003
PubMed
Summary

Transient Receptor Potential (TRP) channels are crucial in cell signaling but their exact roles in complex processes remain unclear. Research is beginning to reveal how TRP channels interact with other proteins in mammalian cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Poly(ADP-ribose) polymerase regulates transient receptor potential channel M2-dependent calpain activation in rd1 mouse retinal degeneration.

Neural regeneration research·2026
Same author

Molecular basis for the sensitivity of TRP channels to polyunsaturated fatty acids.

Naunyn-Schmiedeberg's archives of pharmacology·2018
Same author

Nanodiscs for INPHARMA NMR Characterization of GPCRs: Ligand Binding to the Human A2A Adenosine Receptor.

Angewandte Chemie (International ed. in English)·2017
Same author

A novel form of capsaicin-modified amygdala LTD mediated by TRPM1.

Neurobiology of learning and memory·2016
Same author

TRPV4 Regulates Breast Cancer Cell Extravasation, Stiffness and Actin Cortex.

Scientific reports·2016
Same author

A CD2AP Mutation Associated with Focal Segmental Glomerulosclerosis in Young Adulthood.

Clinical medicine insights. Case reports·2016

Area of Science:

  • Molecular and Cellular Biology
  • Neuroscience
  • Physiology

Background:

  • Transient Receptor Potential (TRP) channels participate in diverse cellular signaling cascades, responding to various stimuli including hormones, neurotransmitters, and physical forces.
  • The precise functional roles of TRP channels in hormonal control, nociception, and calcium homeostasis are not fully understood due to their involvement in complex macromolecular structures.
  • While the role of Drosophila TRP in phototransduction is increasingly clear, the integration of mammalian TRP channels into signal transduction complexes is an emerging area of research.

Purpose of the Study:

  • To explore the interactions of TRP channels with other proteins, particularly PDZ domain proteins and calcium-binding proteins, in mammalian cells.
  • To elucidate the potential scaffolding and regulatory functions of protein interactions involving TRP channels.

Related Experiment Videos

  • To investigate the dynamic nature of TRP channel interactions and their implications for cellular signaling.

    • Main Methods:

    • Review and synthesis of existing literature on TRP channel interactions.
    • Analysis of reported protein-protein interactions, including those with PDZ domain proteins (e.g., INAD, NHERF) and calcium-binding proteins (e.g., calmodulin).
    • Discussion of experimental evidence regarding the functional consequences of these interactions on TRP channel regulation.

    Main Results:

    • TRP channels form stable complexes with PDZ domain proteins, which can act as scaffolds and regulators, as exemplified by INAD in Drosophila.
    • In mammalian cells, interactions between NHERF and TRPC4 suggest a role for PDZ proteins in modulating TRP channel activity, potentially affecting store-dependent calcium regulation.
    • Interactions with calcium-binding proteins like calmodulin are calcium-dependent and dynamic, but their natural role with TRP channels remains an open question.

    Conclusions:

    • TRP channels engage in complex protein interactions that are critical for their function in cellular signaling.
    • PDZ domain proteins play a significant role in organizing and regulating TRP channel complexes in both invertebrates and mammals.
    • Further research is needed to fully understand the dynamic interactions of TRP channels with calcium-binding proteins and their physiological relevance.