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The TRPV6 gene, cDNA and protein.
Daniela Hirnet1, Jenny Olausson, Claudia Fecher-Trost
1Experimentelle und Klinische Pharmakologie und Toxikologie, Universität des Saarlandes, D 66421 Homburg, Germany.
Cell Calcium
|May 27, 2003
Summary
Mouse TRPV6, a calcium channel protein, is found in the pancreas and placenta. This gene encodes a 727-amino acid protein that binds calmodulin in a calcium-dependent manner.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- The Transient Receptor Potential Vanilloid 6 (TRPV6) gene encodes a calcium-permeable ion channel crucial for calcium homeostasis.
- Understanding the characteristics and expression patterns of mouse TRPV6 provides insights into its physiological roles.
Purpose of the Study:
- To characterize the mouse TRPV6 gene and its encoded protein.
- To investigate the expression profile of TRPV6 in various mouse tissues.
Main Methods:
- Gene localization and size determination.
- Protein characterization including amino acid count and molecular mass.
- Antibody validation for protein detection.
- Calmodulin binding assays in the presence and absence of calcium.
- Transcript expression analysis using poly(A)+RNA from various tissues.
Main Results:
- The mouse TRPV6 gene is located on chromosome 6, spanning 15.66kb.
- The encoded protein has 727 amino acids, a molecular mass of 83,210Da, and is glycosylated.
- Mouse TRPV6 binds calmodulin in a Ca2+-dependent manner, similar to human TRPV6.
- TRPV6 transcripts are abundant in pancreas and placenta, with lower levels in stomach and kidney; absent in heart, brain, intestine, esophagus, and aortic endothelial cells.
Conclusions:
- Mouse TRPV6 exhibits distinct tissue-specific expression patterns, predominantly in the pancreas and placenta.
- The Ca2+-dependent calmodulin binding suggests a role in calcium signaling pathways within these tissues.