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Chromatographic separation of Lewis a and Lewis x oligosaccharides as glycosynthons

Natacha Frison1, Laurent Meunier, Philippe Marceau

  • 1Vectorisation et Trafic Intracellulaire, Glycobiologie, Centre de Biophysique Moléculaire, Centre National de la Recherche scientifique, rue Charles-Sadron, 45071 Orleans cedex 2, France.

Biochimie
|May 27, 2003
PubMed

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Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...