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Updated: Aug 18, 2026
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
Published on: December 3, 2017
Induction of the differentiation of lentoids from primate embryonic stem cells
Sotaro Ooto1, Masatoshi Haruta, Yoshihito Honda
1Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Purpose:
To produce lens cells from primate embryonic stem (ES) cells in a reproducible, controlled manner.
Methods:
Cynomologus monkey ES cells were induced to differentiate by stromal cell-derived inducing activity (SDIA). The lentoids produced by this treatment were processed for immunohistochemical and immunoblotting analysis. The effect of varying the concentration of fibroblast growth factor (FGF)-2 and the density of the ES colonies plated during the differentiation process were also examined.
Results:
After a 2- to 3-week induction period, lentoids were produced by a subpopulation of ES colonies. Western blot analysis and immunohistochemistry revealed that these lentoids expressed alphaA-crystallin and Pax6. The number of lentoids resulting from treatment increased with increasing FGF-2 concentration and plated colony density.
Conclusions:
The differentiation of primate ES cells into lentoids can be achieved by treatment with SIDA. ES cells can be used to facilitate a greater understanding of the mechanisms functioning in differentiation in vivo and in vitro.
