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Related Experiment Videos

Microsatellite instability and p53 expression in gallbladder carcinomas.

Fausto Sessa1, Daniela Furlan, Anna Genasetti

  • 1Department of Clinical and Biological Sciences, University of Insubria, Varese, Italy.

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
|May 27, 2003
PubMed
Summary

Microsatellite instability does not appear to be involved in gallbladder cancer (GC) development. However, p53 protein alterations are common in most GC types, suggesting its significant role in gallbladder tumorigenesis.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Gastroenterology

Background:

  • Gallbladder cancer (GC) is a significant malignancy with varied histological subtypes.
  • Understanding the molecular alterations, such as microsatellite instability (MSI) and p53 expression, is crucial for elucidating GC pathogenesis.

Purpose of the Study:

  • To investigate the microsatellite instability (MSI) status in a series of gallbladder cancers (GCs).
  • To evaluate the expression of p53 protein and its correlation with histological type in GCs.
  • To examine the expression of DNA mismatch repair proteins (hMSH2, hMLH1) in GCs.

Main Methods:

  • Analysis of 71 gallbladder cancer (GC) specimens of diverse histological types.
  • Microsatellite analysis using the BAT-26 mononucleotide locus to determine MSI status.

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  • Immunohistochemical studies for p53 protein, hMSH2, and hMLH1 expression, alongside differentiation markers.

    • Main Results:

    • All 71 studied GCs were found to be microsatellite stable (MSS).
    • p53 protein expression was detected in 100% of undifferentiated GCs, 67% of conventional adenocarcinomas, 50% of mucinous adenocarcinomas, and 20% of squamous differentiation GCs.
    • All MSS tumors exhibited immunohistochemical expression of both hMSH2 and hMLH1.

    Conclusions:

    • Microsatellite instability (MSI) does not appear to play a role in the development of gallbladder cancer (GC).
    • p53 protein alterations are a significant finding in a large proportion of GCs, particularly undifferentiated and conventional adenocarcinomas.
    • p53 alterations are less frequent in mucinous and squamous cell carcinomas of the gallbladder.