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Related Experiment Videos

Continuous annular chromatography.

Frank Hilbrig1, Ruth Freitag

  • 1Center of Biotechnology, Faculty of Basic Sciences, Swiss Federal Institute of Technology Lausanne, 1015, Ecublens, Switzerland.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|May 28, 2003
PubMed
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Continuous annular chromatography (CAC) offers a powerful method for separating complex mixtures, particularly in preparative bioseparation. This review explores its applications, instrumentation, and scale-up potential for isolating biomolecules.

Area of Science:

  • Chromatography
  • Bioseparation
  • Chemical Engineering

Background:

  • Continuous annular chromatography (CAC) is a chromatographic technique known for decades.
  • It enables continuous separation of multi-component and bi-component mixtures.
  • CAC transforms one-dimensional batch separation into a two-dimensional continuous process.

Purpose of the Study:

  • To review the capacity of continuous annular chromatography (CAC) for preparative bioseparation.
  • To discuss historical developments, theoretical predictions, and simulations of CAC separations.
  • To provide an overview of current CAC instrumentation and applications in biomolecule isolation.

Main Methods:

  • Review of historical developments and predecessors of modern CAC.
  • Discussion of theoretical prediction and simulation of CAC separations.

Related Experiment Videos

  • Overview of current CAC instrumentation and applications, focusing on protein and biomolecule isolation.

    • Main Results:

    • CAC has been applied to various chromatographic modes, excluding linear gradient elution.
    • The review covers theoretical advancements, instrumentation, and practical applications of CAC.
    • Scale-up, method development, and transfer from batch to continuous CAC are discussed with examples.

    Conclusions:

    • Continuous annular chromatography (CAC) is a versatile technique for preparative bioseparation.
    • It offers advantages for isolating proteins and other biomolecules, with considerations for scale-up.
    • Further development and application of CAC are crucial for advancing bioseparation processes.