Related Experiment Video
Updated: Aug 18, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 12, 2014
[The study of p16 gene mutation and methylation in laryngeal squamous cell carcinoma]
1Department of Otorhinolaryngology, Second Clinical College, Chinese Medical University, Shenyang 110003, China. baisu@pub.sy.ln.cn
Objective:
To investigate the development and mechanism of the p16 gene inactivation in laryngeal squamous cell carcinoma (LSCC).
Methods:
With PCR-based methylation assay and PCR-SSCP, first and second exon mutation in p16 gene and methylation of endogenase in promoter in 32 cases of LSCC and paracancer tissue were studied.
Results:
No mutation were found in first and second exon in the samples; methylation of Cfo I were detected in 6 cases in first exon, methylation of Sac II was found in 4 cases, and methylation of Hpa II in 4 cases. In second exon, methylation of Hpa II was detected in 5 cases and methylation of Cfo I in 6 cases.
Conclusion:
Methylation of promoter in p16 gene is one of the important mechanisms of the inactivation of the gene. The inactivation of p16 gene has a close relation with the development and progress of LSCC.
More Related Videos
07:43Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
Published on: July 29, 2017
07:50Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020