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[Study on the relationship between the abnormal expression of c-myc and multidrug resistance in KB cell lines]

Y He1, J Zhang, J Zhang

  • 1Department of Otorhinolaryngolgy, Nanfang Hospital, First Military Medical University, Guangzhou 510510, China. ying-h@163.net

Zhonghua Er Bi Yan Hou Ke Za Zhi
|May 29, 2003
PubMed
Abstract

Insights

c-Myc protein expression is closely linked to multidrug resistance (MDR) and regulates the MDR1 gene. Targeting c-Myc may offer a new strategy for gene therapy in proliferative diseases with MDR.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Therapy

Background:

  • c-Myc is a proto-oncogene often overexpressed in proliferative diseases.
  • Multidrug resistance (MDR) is a major challenge in cancer treatment, often mediated by P-glycoprotein (P-gp) encoded by the MDR1 gene.
  • The relationship between c-Myc and MDR1 regulation remains incompletely understood.

Purpose of the Study:

  • To investigate the relationship between c-Myc expression and MDR1 regulation.
  • To explore gene therapy strategies for proliferative diseases characterized by c-Myc overexpression and MDR phenotype.

Main Methods:

  • Immunohistochemistry and flow cytometry were used to detect c-Myc protein and P-gp expression in KB cell lines.
  • KB cell lines, including multidrug-resistant KBv and sensitive KB, were treated with anti-mdr1-ribozyme.

Main Results:

  • KB cell lines express c-Myc protein, with higher levels observed in multidrug-resistant KBv cells compared to sensitive KB cells.
  • Treatment with anti-mdr1-ribozyme reduced c-Myc protein levels in both KBv/5mR3 and KB/5mR3 cell lines.
  • P-gp expression decreased in KBv/5mR3 cells post-treatment but showed no significant change in KB/5mR3 cells.

Conclusions:

  • A strong correlation exists between Myc protein and the MDR phenotype.
  • c-myc plays a role in regulating the expression of mdr1.
  • Myc protein can serve as a novel biomarker for monitoring the development of MDR.

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