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DNA ploidy in cervical lesions assessed by computed image analysis: relation to histopathology
N K Shirata1, M C Nogueira Zerbini, A Longatto Filho
1Division of Pathology, Adolfo Lutz Institute, Avenida Dr. Arnaldo 355, Cerqueira Cezar 05025-902, São Paulo, SP, Brazil. shirata86@hotmail.Com
Objective:
To assess the different patterns of DNA nuclear content in cervical intraepithelial neoplasia.
Study Design:
DNA nuclear content patterns (DNA ploidy) were assessed in 82 paraffin embedded samples diagnosed as chronic cervicitis CIN 1, CIN 2 and CIN 3 by image analysis using the CAS200 BD System. These results were compared to the consensus diagnosis of two pathologists. The chi-square test was used to evaluate the results of classificatory variables (CIN 1, CIN 2 and CIN 3).
Results:
Aneuploidy was found in 86% of CIN 3; 59% CIN 2; 26%% CIN 1. All samples classified as chronic cervicitis were diploid.
Conclusions:
The DNA nuclear content was found to parallel the three grades of the histological WHO classification. Aneuploidy was strongly associated with the high-grade lesions; the detection of a subset aneuploidy of low grade lesions would suggest a indicate closer follow-up of these cases.