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Related Experiment Videos

Ultrastructural changes in the developing nucleolus following axotomy.

N B Kinderman, A LaVelle

    Brain Research
    |May 28, 1976
    PubMed
    Summary

    A large intranucleolar body, composed of ribonucleoprotein particles, develops in hamster facial motor neurons. Axotomy of the facial nerve prevents its formation, suggesting a role in rRNA synthesis.

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Developmental Biology

    Background:

    • Nucleoli are essential for ribosome biogenesis.
    • Intranucleolar bodies have been observed in specific neuronal populations.
    • The function of intranucleolar bodies remains largely unknown.

    Purpose of the Study:

    • To investigate the development and response of intranucleolar bodies in golden hamster facial motor neurons.
    • To determine the relationship between intranucleolar bodies and facial nerve integrity.
    • To explore the potential role of intranucleolar bodies in rRNA synthesis.

    Main Methods:

    • Electron microscopy was used to examine nucleolar morphology in golden hamster facial motor neurons.
    • Facial nerve axotomy was performed at different postnatal ages.
    • Nucleoli were analyzed at various time points post-axotomy and during development.

    Main Results:

    • A large, basophilic, Feulgen-negative intranucleolar body, composed of ribonucleoprotein granules, was identified.
    • This structure developed between 20-24 days postnatal and reached up to 2 µm in diameter in mature neurons.
    • Facial nerve axotomy at 15 or 20 days prevented the normal formation of the intranucleolar body, and axotomy in adults caused its dispersal.

    Conclusions:

    • The formation and integrity of intranucleolar bodies are dependent on facial nerve integrity.
    • Changes in intranucleolar body morphology correlate with developmental stages and nerve injury.
    • These findings suggest that intranucleolar bodies are indicators of altered ribosomal RNA (rRNA) synthesis in facial motor neurons.

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