Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Relationships between polydnavirus genomes and viral gene expression.

L Cui1, B A. Webb

  • 1Department of Entomology, University of Kentucky, Lexington, USA

Journal of Insect Physiology
|May 29, 2003
PubMed
Summary

Polydnaviruses exhibit unique segmented DNA genomes and divergent gene expression critical for their mutualistic relationship with parasitic wasps. Genome organization, specifically nested DNA segments, correlates with differential gene expression levels in lepidopteran hosts.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Species of sandflies and prevalence of <i>Leishmania</i> infections in sandflies in selected areas of northern and northwestern China].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2026
Same author

Exhaled breath condensate metabolomics reveals conserved networks and bidirectional crosstalk in asthma and CRSwNP.

Rhinology·2026
Same author

[Gross classification of gallbladder cancer with primary lesion limited to the gallbladder wall and its correlation with prognosis and precancerous lesions].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2026
Same author

[Effect of CD4<sup>+</sup>T cell-specific methyltransferase-like 3 gene knockout on calcipotriol-induced atopic dermatitis in mice].

Zhonghua yi xue za zhi·2025
Same author

[Global epidemiology and control strategies of Chikungunya virus: a review].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2025
Same author

[A case of bilateral chylous aqueous humor].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2025

Area of Science:

  • Virology
  • Insect Pathology
  • Genomics

Background:

  • Polydnaviruses possess atypical segmented DNA genomes, uniquely essential for the survival of both the virus and its parasitic Hymenoptera host.
  • These viruses replicate asymptomatically in wasps but disrupt lepidopteran host physiology without replicating their DNA.
  • Viral gene expression is host-specific, with distinct functions in wasps versus lepidopteran hosts.

Purpose of the Study:

  • To investigate the relationship between polydnavirus genome organization and differential gene expression in insect hosts.
  • To understand how viral gene expression contributes to the disruption of lepidopteran host physiology.

Main Methods:

  • Cloning, mapping, and sequence analysis of selected polydnavirus DNA segments.
  • Studying the expression and regulation of viral cys-motif genes involved in immune suppression.

Related Experiment Videos

  • Analyzing the physical organization of viral gene segments, including nested structures.

    • Main Results:

    • Identified nested viral DNA segments encoding abundantly expressed, secreted cys-motif genes.
    • Observed that non-nested segments encode less abundant, intracellularly targeted genes.
    • Established a correlation between segment nesting and gene expression levels.

    Conclusions:

    • The nesting of DNA segments in polydnavirus genomes is linked to differential gene expression levels.
    • The unique genome segmentation may facilitate divergent viral gene expression required for host manipulation in the absence of viral DNA replication.