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Related Experiment Videos

Mouse taste preference tests: why only two bottles?

Michael G Tordoff1, Alexander A Bachmanov

  • 1Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104-3308, USA. tordoff@monell.org

Chemical Senses
|May 29, 2003
PubMed
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Taste preference studies using multiple bottles show that increasing the number of taste solution bottles significantly impacts intake and preference. Three-bottle tests offer greater sensitivity for discerning strain differences in mice.

Area of Science:

  • Behavioral Neuroscience
  • Sensory Science
  • Animal Behavior

Background:

  • Two-bottle preference tests are standard for assessing taste and nutrient solution preferences.
  • Limited research exists on preference tests involving more than two bottles.

Purpose of the Study:

  • To compare the sensitivity and results of two-bottle preference tests with three- and six-bottle tests.
  • To investigate the influence of the number of bottles on taste solution intake and preference.

Main Methods:

  • Experiment 1: Compared two-, three-, and six-bottle tests using 129X1/SvJ (129) and C57BL/6J (B6) mice with saccharin, citric acid, quinine hydrochloride, and NaCl solutions.
  • Experiment 2: Assessed NaCl preferences in six-bottle tests with varying numbers of NaCl solution bottles.
  • Follow-up experiment: Measured intake via volume and licks to rule out spillage as a factor.

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Main Results:

  • Mice consumed more taste solution in three-bottle tests (one taste, two water) compared to standard two-bottle tests.
  • Three-bottle tests revealed strain-specific side preferences and were more sensitive in distinguishing between 129 and B6 mouse strains.
  • NaCl preference showed a monotonic relationship with the number of available NaCl spouts in six-bottle tests.
  • Intake measures (volume and licks) yielded similar results, indicating spillage did not account for intake variations.

Conclusions:

  • The number of taste solution and water bottles profoundly influences taste solution intake and preference.
  • Three-bottle preference tests may offer enhanced sensitivity over traditional two-bottle tests for certain research applications.