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[Effects of mtDNA deletion associated with abnormal expression in rat cochlear with presbycusis]
Haiyan Wu1, Baodong Du, Ping Wang
1Department of Otorhinolaryngology, First Hospital, Jilin University, Changchun 130021, China. wuhaiyan72@perfectmail.com
Objective:
To investigate the effects of the common deletion (4834-bp) of mitochondrial DNA (mtDNA) in rat cochlear with presbycusis.
Methods:
The mtDNA 4834-bp deletion was analyzed by PCR. The mitochondrial-encoded cytochrome c oxidase subunit I (COXI) transcript level and cytochrome c oxidase (COX) activity were measured by RT-PCR and histochemical methods respectively.
Results:
The 4834-bp deletion occurred in all the senescent rat cochlear. The COXI transcript level was decreased associated with the decline of COX activity.
Conclusion:
The mtDNA 4834-bp deletion presented in the rat cochlear with presbycusis, which lead to the decrease of COXI transcript level and COX activity, may play an important role in the pathogens of presbycusis.
Insights
A common mitochondrial DNA deletion (4834-bp) was found in aging rat cochlear (presbycusis). This deletion reduced cytochrome c oxidase subunit I (COXI) transcript levels and enzyme activity, suggesting a role in hearing loss.
Area of Science:
- Mitochondrial genetics
- Auditory neuroscience
- Aging research
Context:
- Presbycusis, or age-related hearing loss, is a growing concern.
- Mitochondrial DNA (mtDNA) deletions are implicated in cellular aging.
- The specific role of mtDNA deletions in cochlear aging is not fully understood.
Purpose:
- To investigate the impact of the 4834-bp mitochondrial DNA deletion on the aging rat cochlea.
- To assess the relationship between this mtDNA deletion and markers of cellular energy production.
Summary:
- The 4834-bp deletion of mitochondrial DNA (mtDNA) was detected in all senescent rat cochleae.
- This deletion correlated with decreased levels of the mitochondrial-encoded cytochrome c oxidase subunit I (COXI) transcript.
- A decline in cytochrome c oxidase (COX) activity was observed alongside reduced COXI transcripts.
Impact:
- The mtDNA 4834-bp deletion is present in presbycusis.
- This deletion leads to reduced COXI transcript levels and COX activity.
- The findings suggest the mtDNA 4834-bp deletion plays a significant role in the pathology of age-related hearing loss.