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[Effect of staphylococcal enterotoxin B on ion electrophysiology and permeability in rabbit maxillary sinus
Tao Liu1, Pingchang Yang, Binquan Wang
1Department of Otorhinolaryngology, First Hospital, Shanxi Medical University, Taiyuan 030001, China. lqy90326@public.ty.sx.cn
Objective:
To study the barrier role of staphylococcal enterotoxin B (SEB) in rabbit maxillary sinus mucosa.
Methods:
Rabbit maxillary sinus mucosal membrane was dissected under a surgical microscope. The short-circuit current (Isc), conductance (G) and its permeability to horseradish peroxidase (HRP) were evaluated by employing Ussing chamber technique.
Results:
The stimulation of SEB enhanced Isc, G and its permeability to HRP of sinus epithelial barrier. The same results were obtained by applying tumor necrosis factor alpha (TNF-alpha) to rabbit maxillary sinus. Increase in TNF-alpha level in sinus mucosa was observed by the stimulation of SEB. Those pathophysiological abnormalities of sinus mucosa were abolished by pretreatment with anti-TNF-alpha antibody.
Conclusion:
The SEB has potential effects on rabbit maxillary sinus mucosal barrier, activates the immune cells in the mucosa, and plays certain roles in maxillary sinusitis.
Insights
Staphylococcal enterotoxin B (SEB) disrupts the rabbit maxillary sinus mucosal barrier by increasing permeability and activating immune responses. This effect is mediated by tumor necrosis factor alpha (TNF-alpha).
Area of Science:
- Immunology
- Otolaryngology
- Microbiology
Context:
- The maxillary sinus mucosa serves as a critical barrier against pathogens.
- Staphylococcal enterotoxin B (SEB) is a potent exotoxin produced by Staphylococcus aureus.
- Understanding SEB's role in sinus mucosa is crucial for managing sinusitis.
Purpose:
- To investigate the impact of staphylococcal enterotoxin B (SEB) on the barrier function of rabbit maxillary sinus mucosa.
- To elucidate the mechanisms underlying SEB-induced changes in sinus mucosa.
Summary:
- SEB stimulation significantly increased short-circuit current (Isc), conductance (G), and horseradish peroxidase (HRP) permeability of the sinus epithelial barrier.
- SEB induced an increase in tumor necrosis factor alpha (TNF-alpha) levels within the sinus mucosa.
- Pretreatment with anti-TNF-alpha antibody effectively reversed the SEB-induced pathophysiological abnormalities.
Impact:
- SEB activation of immune cells and disruption of the mucosal barrier contribute to maxillary sinusitis pathogenesis.
- Targeting TNF-alpha may offer a therapeutic strategy for SEB-mediated sinus inflammation.