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Related Experiment Videos

Proliferative activity in periapical lesions.

Teresa Roberta Tripi1, Antonio Bonaccorso, Ernesto Rapisarda

  • 1Cattedra di Odontoiatria Conservatrice, Corso di Laurea in Odontoiatria e Protesi Dentaria, Catania University, Italy.

Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc
|May 30, 2003
PubMed
Summary

Proliferative cell nuclear antigen (PCNA) and Ki67 indicate cell proliferation in periapical lesions. These markers, along with CD3, were highly expressed, while p53 was negative in granulomas and radicular cysts.

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Area of Science:

  • Oral pathology
  • Immunohistochemistry
  • Cell biology

Background:

  • Periapical lesions, such as granulomas and radicular cysts, exhibit varying biological behaviors.
  • Assessing cellular proliferation is key to understanding lesion activity.
  • Markers like Proliferant Cell Nuclear Antigen (PCNA), Ki67, CD3, and p53 are utilized for evaluating proliferative potential.

Purpose of the Study:

  • To investigate the expression of PCNA, Ki67, CD3, and p53 in periapical granulomas and radicular cysts.
  • To correlate the expression of these markers with the proliferative activity of periapical lesions.

Main Methods:

  • Analysis of 16 periapical granulomas and 8 radicular cysts.
  • Semi-quantitative assessment of antigen expression using a scoring system (0-3) based on staining intensity.

Related Experiment Videos

  • Immunohistochemical evaluation for PCNA, Ki67, CD3, and p53.

    • Main Results:

    • Ki67 positive cells were detected in all periapical lesions, notably in pathological keratinic cells.
    • PCNA positive cells were present in 22 out of 24 cases.
    • CD3 reactivity was highly positive, whereas oncoprotein p53 showed negative expression in these lesions.

    Conclusions:

    • Ki67 and PCNA expression are reliable indicators of cell proliferation in periapical lesions.
    • Chronic irritative stimuli likely drive the observed cell proliferation.
    • CD3 positivity suggests an inflammatory component, while p53 negativity indicates no significant oncogenic activity.