Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antibiotic glycosyltransferases.

C T Walsh1, H C Losey, C L Freel Meyers

  • 1Department of Biological Chemistry & Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA. christopher_walsh@hms.harvard.edu

Biochemical Society Transactions
|May 30, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural characterization of enterobactin hydrolase IroE.

Biochemistry·2006
Same author

NovJ/NovK catalyze benzylic oxidation of a beta-hydroxyl tyrosyl-S-pantetheinyl enzyme during aminocoumarin ring formation in novobiocin biosynthesis.

Biochemistry·2005
Same author

Natural product biosynthetic assembly lines: prospects and challenges for reprogramming.

Ernst Schering Research Foundation workshop·2005
Same author

Chain termination steps in nonribosomal peptide synthetase assembly lines: directed acyl-S-enzyme breakdown in antibiotic and siderophore biosynthesis.

Chembiochem : a European journal of chemical biology·2002
Same author

Glycopeptide antibiotic biosynthesis: enzymatic assembly of the dedicated amino acid monomer (S)-3,5-dihydroxyphenylglycine.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Substrate recognition and selection by the initiation module PheATE of gramicidin S synthetase.

Journal of the American Chemical Society·2001
Same journal

Beyond the scaffold: extracellular matrix uptake in breast cancer.

Biochemical Society transactions·2026
Same journal

TDP-43 proteinopathy as a biomarker and therapeutic target in amyotrophic lateral sclerosis.

Biochemical Society transactions·2026
Same journal

Advancing the monitoring of organelle contact sites in vitro and in vivo.

Biochemical Society transactions·2026
Same journal

Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.

Biochemical Society transactions·2026
Same journal

Replication associated nuclear DNA mismatch repair across kingdoms.

Biochemical Society transactions·2026
Same journal

Phosphatases of regenerating liver downregulate PTEN to promote tumorigenesis.

Biochemical Society transactions·2026
See all related articles

This study explores antibiotic glycosylation pathways, focusing on vancomycin and novobiocin biosynthesis. It details the roles of specific glycosyltransferases in attaching sugar moieties to antibiotic scaffolds, crucial for their maturation and function.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Microbiology

Background:

  • Antibiotic biosynthesis often involves late-stage glycosylation of aglycone scaffolds by specific glycosyltransferases.
  • Understanding these enzymatic pathways is crucial for antibiotic development and combating resistance.

Purpose of the Study:

  • To examine the glycosylation pathways in vancomycin and novobiocin antibiotic biosynthesis.
  • To discuss the specificity and mechanisms of the involved glycosyltransferases.

Main Methods:

  • Analysis of sequential glycosylation steps in vancomycin and chloroeremomycin assembly.
  • Examination of enzyme involvement in novobiocin biosynthesis, including L-noviose transfer, methylation, and carbamoylation.
  • Initial characterization of key enzymes NovM and NovP.

Related Experiment Videos

Main Results:

  • Detailed the sequential glucosylation and vancosaminylation by GtfE and GtfD in vancomycin maturation.
  • Described the distinct glycosylation steps by GtfB, GtfA, and GtfC in chloroeremomycin assembly.
  • Outlined the proposed sequential action of NovM, NovP, and NovN in novobiocin biosynthesis.

Conclusions:

  • Glycosyltransferases play critical roles in the structural diversity and maturation of antibiotics.
  • The elucidated pathways provide insights into the biosynthesis of important antibiotic classes.
  • Further characterization of enzymes like NovM and NovP will enhance our understanding of antibiotic synthesis.