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Updated: Aug 9, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Published on: September 30, 2016
Pti1p and Ref2p found in association with the mRNA 3' end formation complex direct snoRNA maturation
Sonia Dheur1, Le Thuy Anh Vo, Florence Voisinet-Hakil
1Institut de Biochimie et Génétique Cellulaires, CNRS, UMR 5095, 1 Rue Camille Saint Saëns, 33077 Bordeaux cedex, France.
Abstract:
Eukaryotic RNA polymerase II transcribes precursors of mRNAs and of non-protein-coding RNAs such as snRNAs and snoRNAs. These RNAs have to be processed at their 3' ends to be functional. mRNAs are matured by cleavage and polyadenylation that require a well-characterized protein complex. Small RNAs are also subject to 3' end cleavage but are not polyadenylated. Here we show that two newly identified proteins, Pti1p and Ref2p, although they were found associated with the pre-mRNA 3' end processing complex, are essential for yeast snoRNA 3' end maturation. We also provide evidence that Pti1p probably acts by uncoupling cleavage and polyadenylation, and functions in coordination with the Nrd1p-dependent pathway for 3' end formation of non-polyadenylated transcripts.
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