Transgenic rabbit producing human growth hormone in milk
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Updated: Sep 25, 2026

Pregnancy and Nursing Management for Embryo-Transferred and Genetically Modified Rabbits
Published on: December 13, 2024
Daniel Lipiński1, Jolanta Jura, Robert Kalak
1Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University of Poznań, ul. Wołyńska 35, 60-637 Poznań, Poland.
Abstract:
The gene construct WAP(6xHisThr):hGH containing the entire human growth hormone gene (hGH) under the rat whey acidic protein (WAP) promoter regulating the expression in mammary glands of mammals was prepared. The 5' end of the gene was modified by the addition of a sequence encoding six histidine residues and a sequence recognized by thrombin. The gene construct was introduced by microinjection into the male pronucleus of a fertilized oocyte. The founder male rabbit was obtained with the transgene mapping to chromosome 7. The presence of the growth hormone was confirmed in samples of milk collected during the lactation of F1 generation females. The growth hormone can be easily purified by affinity chromatography and cleavage by thrombin to an active form.
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