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[The changes of some chemical components in banxiaxiexintang decoction of different combinations]
Tian-xuan Duan1, Chang-hua Ma, Tao Chen
1College of Chinese Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Objective:
The contents of bererine, palmatine and glycyrrhizin acid in Banxiaxiexintang decoction of different combinations were determined by PR-HPLC.
Method:
A Shim-pack CLC-ODS column was used with a mobile phase of CH3CN-H2O (31:69; 0.005 moL.L-1 -pentanesulfonic acid sodium salt, H3PO4: pH 3.0) for bererine andpalmatine, which were detected at the wavelength of 275 nm. A YWG-C18 column was used with a mobile phase of CH3OH-H2O-HAc(62:37:1) for glycyrrhizin acid which was detected at the wavelength of 260 nm.
Result:
Each herbs' combination influences the contents of the 3 components.
Conclusion:
The experiment is an attempt to study the comical foundation of traditional Chinese prescription.
