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[Measurement of baicalin contents by HPLC]
Zhu-ying He1, Pei-xue Cao, Guang-yi Liang
1First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China.
Objective:
To compare the contents of baicalin in the raw medical material and the different prepared medical materials of Scutellaria baicalensis.
Method:
The contents of baicalin were determined by HPLC. Chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of methanol-0.04% phosphoric acid (46:54). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.0280-4.6700 micrograms with correlation coefficient 1.0000. The average recovery of baicalin was 101.22% with RSD = 2.54% (n = 5).
Result:
The contents of baicalin were 6.80% in raw material, 6.00% and 6.73% in the different prepared medical materials of S. baicalensis respectively.
Conclusion:
The two different kinds of preparing methods can be used to the raw material of S. baicalensis.