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[Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae]
Xiao-jun Song1, Yong-xin Zhang, Ying Zhang
1First People' Hospital of Hangzhou, Hangzhou 310006, Zhejiang, China.
Objective:
To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.
Method:
TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (15:85) as mobile phase. The detection wavelength was 327 nm.
Result:
The qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods.
Conclusion:
The methods and data could be used for quality control.
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