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[Structural features of a neutral heteropolysaccharide CPB-4 from Cynanchum paniculatum]
Shun-chun Wang1, Xing-feng Bao, Ji-nian Fang
1Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Objective:
To study the chemical features of CPB-4, a heteropolysaccharide obtained from Cynanchum paniculatum.
Method:
Sugar composition analysis, methylation analysis, partial hydrolysis and carbon-13 nuclear magnetic resonance were used to determine the sugar composition, linkages, main chain, branch chains and branching points.
Result:
CPB-4 is composed of L-arabinose, L-xylose, L-rhamnose and D-galactose in closely molar ratios of 0.8:0.2:0.2:1.0. Its main chain is comprised of 1, 5 linked galactose and side chains are comprised of terminal xylose, terminal arabinose, oligosaccharide of arabinose and oligosaccharide of arabinose, rhamnose and galactose. The branching points are located at C-6 and C-2 of galactose.
Conclusion:
CPB-4 is a new heteropolysaccharide from C. paniculatum.