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[Determination of gossypol in cotton root bark by HPLC]
Guang-hong Cui1, Jia-chun Chun, Da-yong Cai
1Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061. guanghongcui@263.net
Abstract
Objective:
To establish a method for the determination of gossypol in cotton root bark.
Method:
Samples were extracted with acetone, and determined on a C18 column with the mobile phase (Acetonitrile-0.2% phosphoric acid, 85:15) and UV-235 nm detector.
Result:
The recovery rate was 97.6%, RSD 1.59% (n = 5).
Conclusion:
This method can be used for the determination of gossypol in cotton root bark and the content of gossypol in three different species has been determined.