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[Determination of trigonelline in Trigonella foenum-graecum by HPLC]
Huai-qing Zhao1, Yan Qu, Xue-ya Wang
1Shenyang Pharmaceutical University, Pharmaceutical College, Shenyang 110015, Liaoning, China.
Abstract
Objective:
A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum.
Method:
The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(75:25) and detection wavelength was set at UV 265 nm.
Result:
The standard curve was linear in the range of 3.68-73.60 micrograms.mL-1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4% and 1.83% (n = 6) respectively.
Conclusion:
It provides scientific indexes for quality control of T. foenum-graecum.