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Updated: Aug 20, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
[A new steroidal glycoside from fermented leaves of Agave americana]
Jian-ming Jin1, Xi-kui Liu, Chong-ren Yang
1State Key Laboratory of Phytochemistry and Western Plant Resources in China.
Objective:
To investigate the constituents of fermented leaves of Agave americana, and discover new compounds.
Method:
Compounds were purified with silica gel and C8 reverse--phase silica gel column chromatography. The structures were elucidated by chemical and spectroscopic evidence.
Result:
Three steroidal compounds were obtained and their structures were identified as (25R)-5 alpha-spirostan-3 beta, 6 alpha, 23 alpha-triol 6-O-beta-D-glucopyranoside(1), (25R)-5 alpha-spirostan-3 beta, 6 alpha, 23 alpha-triol-3, 6-di-O-beta-D-glucopyranoside (cantalasaponin-1) (2) and (25R)-5 alpha-spirostan-3 beta, 6 alpha, 23 alpha-triol(hongguanggenin) (3).
Conclusion:
Compound 1 is new compound, named agamenoside C.
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