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Expression of apoptosis-associated protein RCAS1 in macrophages of histiocytic necrotizing lymphadenitis

Yasunobu Abe1, Koichi Ohshima, Manabu Nakashima

  • 1Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. abey@intmed3.med.kyushu-u.ac.jp

International Journal of Hematology
|May 31, 2003
PubMed

Insights

Receptor-binding cancer antigen (RCAS1) on macrophages is elevated in histiocytic necrotizing lymphadenitis. This suggests RCAS1 may induce T-cell apoptosis in this condition.

Area of Science:

  • Immunology
  • Oncology
  • Pathology

Background:

  • Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is found on various cancer cells.
  • RCAS1 is an apoptosis-associated protein affecting T-cells and erythroid progenitor cells.
  • Monocytes/macrophages have been previously shown to express RCAS1.

Purpose of the Study:

  • To investigate RCAS1 expression in histiocytic necrotizing lymphadenitis (HNL).
  • To determine the role of RCAS1 in HNL pathogenesis, particularly T-cell apoptosis.

Main Methods:

  • Immunohistochemical staining using the 22-1-1 monoclonal antibody (MoAb) was performed.
  • RCAS1 expression was analyzed in 9 HNL cases and 9 reactive lymphadenitis controls.
  • The ratio of RCAS1+ cells to CD68+ cells (monocytes/macrophages) was quantified.

Main Results:

  • RCAS1 expression was significantly higher in HNL cases compared to controls.
  • The ratio of RCAS1+ cells to CD68+ cells was significantly elevated in HNL (P = .0002).

Conclusions:

  • RCAS1 is expressed on macrophages in HNL.
  • Macrophages expressing RCAS1 may contribute to the induction of T-cell apoptosis in HNL.

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