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No evidence for a putative involvement of platelet-activating factor in systemic lupus erythematosus without active
Yves Denizot1, Eric Liozon, Laurence Guglielmi
1UMR CNRS 6101, Laboratoire d'Immunologie, Faculté de Médecine, 2 rue Dr Marcland, 87025 Limoges, France. yves.denizot@unilim.fr
Background:
Platelet-activating factor (PAF) seems to be implicated in systemic lupus erythematosus (SLE) patients with associated renal diseases.
Aims:
In this study, we ensured the role of PAF in SLE patients without renal complications.
Methods:
Blood PAF and acetylhydrolase activity, plasma soluble phospholipase A(2), and the presence of antibodies against PAF were investigated in 17 SLE patients without active nephritis and in 17 healthy controls.
Results:
Blood PAF levels were not different (p=0.45) between SLE patients (6.7+/-2.8 pg/ml) and healthy subjects (9.6+/-3.1 pg/ml). Plasma acetylhydrolase activity (the PAF-degrading enzyme) was significantly (p=0.03) elevated in SLE patients (57.8+/-6.4 nmol/min/ml) as compared with controls (37.9+/-2.6 nmol/min/ml). Plasma soluble phospholipase A(2) (the key enzyme for PAF formation) was not different (p=0.6) between SLE patients (59.1+/-5.1 U/ml) and controls (54.7+/-2.4 U/ml). Antibodies against PAF were detected only in 3/17 SLE patients. Flow cytometry analysis did not highlight PAF receptors on circulating leukocytes of SLE patients.
Conclusion:
This clinical study highlights no evidence for a putative important role of PAF in SLE patients without active nephritis.