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Low doses of diagnostic energy X-rays protect against neoplastic transformation in vitro
J L Redpath1, Q Lu, X Lao
1Department of Radiation Oncology, College of Medicine, University of California Irvine, 92697, USA. jlredpat@uci.edu
Purpose:
To investigate the effect of low doses of 60 kVp X-rays on in vitro transformation frequency.
Materials And Methods:
HeLa x skin fibroblast human hybrid cells were used to assay transformation from the non-tumorigenic to the tumorigenic phenotype. Subconfluent cultures of cells were exposed to a range of doses of 60 kVp X-rays and seeded for assay of transformation after 24 h post-irradiation holding. Experiments were repeated at least three times and the data pooled for analysis. Transformation frequencies were compared with those of sham-irradiated controls.
Results:
At doses < 1 cGy, the observed transformation frequencies were significantly less than those seen in unirradiated cells.
Conclusion:
Low doses (< 1 cGy) of 60 kVp X-rays protect HeLa x skin fibroblast human hybrid cells against neoplastic transformation in vitro.