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Bioterrorism and electron microscopic differentiation of poxviruses from herpesviruses: dos and don'ts

Sara E Miller1

  • 1Duke University Medical Center, Durham, North Carolina 27710, USA. sara.miller@duke.edu

Ultrastructural Pathology
|May 31, 2003
PubMed

Insights

Electron microscopy rapidly differentiates smallpox virus from herpesviruses, crucial for biodefense readiness. Laboratories must assess expertise, biohazard capacity, and staff health before monitoring potential smallpox cases.

Area of Science:

  • Virology
  • Biodefense
  • Microscopy

Background:

  • The threat of terrorism necessitates enhanced biodefense readiness.
  • Smallpox virus is a potential bioterrorism agent.
  • Herpesviruses can cause skin lesions resembling early smallpox infection.

Purpose of the Study:

  • To highlight the importance of accurate viral identification in biodefense.
  • To present electron microscopy as a key tool for differentiating poxviruses and herpesviruses.
  • To outline essential considerations for laboratories involved in smallpox surveillance.

Main Methods:

  • Electron microscopy (EM) for rapid viral differentiation.
  • Assessment of laboratory capabilities for biohazard handling.
  • Evaluation of laboratory staff health and immune status.

Main Results:

  • Electron microscopy provides a fast and reliable method to distinguish between poxviruses and herpesviruses.
  • Laboratories require specific expertise in virus identification.
  • Capacity for handling biohazardous materials is critical.

Conclusions:

  • Electron microscopy is vital for identifying smallpox virus in potential bioterrorism scenarios.
  • Laboratories must be prepared with the necessary infrastructure and personnel.
  • Staff health and safety are paramount for effective biodefense operations.

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