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Related Experiment Videos

Period gene expression in mouse endocrine tissues.

Eric L Bittman1, Leo Doherty, Liyue Huang

  • 1Dept. of Biology, Program in Neuroscience, and Center for Neuroendocrine Studies, Univ. of Massachusetts, Amherst, MA 01003, USA. elb@bio.umass.edu

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|May 31, 2003
PubMed
Summary

The Period (Per) genes, crucial for circadian rhythms, are expressed in peripheral endocrine glands like the adrenal, thyroid, and testis. This study maps their mRNA and protein localization in these tissues, revealing tissue-specific patterns.

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Area of Science:

  • Chronobiology
  • Molecular Biology
  • Endocrinology

Background:

  • Circadian rhythms are regulated by oscillating Period (Per) genes.
  • Per genes are expressed in both central and peripheral tissues.
  • Hormones are suspected to coordinate physiological functions temporally.

Purpose of the Study:

  • To investigate the expression of mPer1 and mPer2 genes in peripheral endocrine tissues.
  • To localize mPer1 and mPer2 mRNA and PER1 protein in adrenal, thyroid, and testis.
  • To understand the temporal and cellular distribution of Per gene expression in peripheral organs.

Main Methods:

  • In situ hybridization to detect mPer1 and mPer2 mRNA.
  • Immunocytochemistry to visualize PER1 protein.

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  • Double labeling with 5'-bromodeoxyuridine to assess cell proliferation and PER1 expression timing.

    • Main Results:

    • mPer1 and mPer2 mRNA were localized in specific compartments of the adrenal gland (medulla and cortex, respectively).
    • mPer1 mRNA was detected in the thyroid gland.
    • mPer1 expression in the testis showed a significant increase in seminiferous tubules during the early night, with PER1 protein found in spermatocytes and spermatids.

    Conclusions:

    • The study confirms the expression of mPeriod genes in peripheral endocrine glands.
    • Peripheral expression patterns suggest a role in tissue-specific circadian regulation.
    • Further research is needed to elucidate central regulation, hormonal control, and the functional significance of these peripheral circadian rhythms.