Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
Evolutionary Processes in Microbes01:26

Evolutionary Processes in Microbes

Microbial evolution occurs rapidly due to short generation times and a variety of genetic processes, including horizontal gene transfer, mutation, recombination, and genetic drift. These mechanisms collectively enable microbes to adapt swiftly to changing environments.Horizontal gene transfer (HGT) allows genes to move between different species and occurs through three main mechanisms: conjugation, transformation, and transduction. Conjugation involves direct cell-to-cell contact for DNA...
Deep Sea Microbial Ecology01:18

Deep Sea Microbial Ecology

The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches extending beyond...
Regulation of Bacterial Virulence01:28

Regulation of Bacterial Virulence

Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of iron uptake systems on cefiderocol activity in <i>Escherichia coli</i>: At the crossroads of antibiotic resistance and virulence.

Antimicrobial agents and chemotherapy·2026
Same author

Children in all policies: lessons from a global collaboration to promote the health and wellbeing of children and future generations.

Lancet (London, England)·2026
Same author

Effects of septic shock vasopressors on the fitness of Escherichia coli.

Scientific reports·2026
Same author

Evaluation of the contribution of trio-exome sequencing in selected prenatal indications.

Frontiers in genetics·2026
Same author

Spontaneous TisB toxin expression modulates <i>Escherichia coli</i> metabolic adaptation during growth transitions.

FEMS microbes·2026
Same author

Genomic diversification, adaptive convergence, and regulatory rewiring in aging Escherichia coli colonies.

BMC microbiology·2026

Related Experiment Video

Updated: Jul 15, 2026

Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

Stress-induced mutagenesis in bacteria.

Ivana Bjedov1, Olivier Tenaillon, Bénédicte Gérard

  • 1INSERM U571, Faculté de Médecine Necker-Enfants Malades, Université ParisV, 156 rue Vaugirard, 75730 ParisCedex 15, France.

Science (New York, N.Y.)
|May 31, 2003
PubMed
Summary

Stress-induced mutagenesis, or mutagenesis in aging colonies (MAC), in E. coli reflects diverse environmental pressures. This process may aid bacterial adaptation and evolution under stress.

More Related Videos

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
06:45

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Published on: January 18, 2014

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
11:37

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Published on: July 3, 2017

Related Experiment Videos

Last Updated: Jul 15, 2026

Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
06:45

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Published on: January 18, 2014

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
11:37

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Published on: July 3, 2017

Area of Science:

  • Evolutionary biology
  • Microbial genetics
  • Bacterial adaptation

Background:

  • Stress-induced mutagenesis (SIM) is a phenomenon where environmental stress increases mutation rates.
  • Understanding SIM's role in evolution is crucial for comprehending bacterial adaptation strategies.

Purpose of the Study:

  • To evaluate the evolutionary significance of stress-induced mutagenesis in natural isolates of Escherichia coli.
  • To investigate the factors influencing mutagenesis in aging colonies (MAC) and its contribution to adaptive evolution.

Main Methods:

  • Studied mutagenesis in aging colonies (MAC) of diverse Escherichia coli natural isolates.
  • Analyzed the dependence of MAC on starvation, oxygen, and specific regulons (RpoS, cAMP).
  • Utilized computer modeling to explore the role of beneficial mutations generated by SIM.

Main Results:

  • A significant proportion of E. coli isolates displayed strong MAC.
  • MAC variability correlated with the diversity of selective pressures in different ecological niches.
  • MAC was found to depend on starvation, oxygen, and RpoS and cAMP regulons.
  • Computer modeling and mutagenesis patterns supported the selection of MAC for generating beneficial mutations.

Conclusions:

  • Stress-induced mutagenesis, potentially a byproduct of survival strategies, plays a role in adaptive evolution.
  • The variability in MAC reflects adaptation to diverse environmental conditions.
  • Stress-induced mutations contribute to bacterial evolution regardless of their origin.