Related Experiment Video
Updated: Aug 7, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
Polar expeditions--provisioning the centrosome for mitosis
Sarah P Blagden1, David M Glover
1Cancer Research UK Cell Cycle Genetics Research Group, University of Cambridge, Department of Genetics, Downing Street, Cambridge, CB2 3EH, UK.
Abstract:
It is now clear that both centrioles and their surrounding pericentriolar material (PCM) are capable of self-assembly. Whereas centrioles are normally duplicated during G1-S phase, PCM components may be loaded onto centrosomes in both a microtubule-dependent and -independent manner at all stages of the cell cycle. Centrosomes enlarge dramatically after mitotic entry, when both Aurora A and Polo-like kinases cooperate to recruit additional gamma-tubulin ring complexes and microtubule-associated proteins to assist spindle formation.
Related Concept Videos
The Mitotic Spindle
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
Centrosome Duplication
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Spindle Assembly
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
The Mitotic Spindle
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
Centrosome Duplication
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrioles and Centrosomes
Near the end of the prophase, also called late prophase or "prometaphase,"...

